Q2 Newsletter Just Released
July 2024: Delve further into the world of noncanonical amino acids with Articles, Highlights, Events and Insights from the scientists and customers of Aralez Bio.
Download the newsletterIOPC June 2024
Tina Boville, Aralez Bio CEO and co-founder, is an invited speaker at this year's event. Her talk is titled, "Expanding the Universe of Noncanonical Amino Acids with Biocatalysis." Contact info@aralezbio.com if you'd like her to send you a copy of the presentation.
Learn moreAralez Bio in the News - April 3, 2024
Aralez Bio was just featured in CHEManager International issue 01/2024. Tina and David were interviewed by this DACH-focused Wiley publication that gives strategic news to the chemical and pharmaceutical industries.
Go to the articleQ1 2024 Newsletter Just Released
Delve further into the world of noncanonical amino acids with Articles, Highlights, Events and Insights from the scientists and customers of Aralez Bio.
Download the newsletterAralez Bio in the Community - Oct. 2023
The City of San Leandro featured our CEO Tina Boville & our Lead Protein Engineer Ella Watkins-Dulaney in their Made in San Leandro: Celebrating Women in Manufacturing series.
Watch the video segmentYear: 2021
Abstract:
Here, we show that ketone-derived enolates can serve as nucleophiles in the TrpB reaction to achieve the asymmetric alkylation of ketones, an outstanding challenge in synthetic chemistry...
Year: 2020
Abstract:
This review examines the long and storied career of TrpS from the perspective of its application in ncAA synthesis and biocatalytic cascades.
Year: 2020
Abstract:
Using a continuous directed evolution platform called OrthoRep, we rapidly evolve the Thermotoga maritima tryptophan synthase β-subunit (TmTrpB)...
Year: 2019
Abstract:
Using an engineered tryptophan synthase β-subunit (TrpB), stereochemically pure AzAla can be synthesized at scale starting from commercially available azulene and L-serine....
Year: 2019
Abstract:
Here we report the use of nitroalkanes as a structurally versatile class of nucleophilic substrates for C−C bond formation catalyzed by variants of the β-subunit of tryptophan synthase (TrpB)...
Year: 2019
Abstract:
Here we employ directed evolution to engineer TrpB to accept 3-substituted oxindoles and form C–C bonds leading to new quaternary stereocenters...
Year: 2018
Abstract:
Here, we show that directed evolution caused a gradual change in the rate-limiting step of the catalytic cycle...
Year: 2018
Abstract:
Here, we describe the engineering of TrpB from Thermotoga maritima that improved synthesis of 4-CN-Trp from 24% to 78% yield...
Year: 2018
Abstract:
Herein we report a stereoselective biocatalytic synthesis of beta-branched tryptophan analogues using PfTrpB7E6...
Year: 2018
Abstract:
...we briefly outline different enzyme engineering strategies and then discuss examples where engineering has generated new ‘ncAA synthases’ for efficient, environmentally benign production...
Year: 2017
Abstract:
We have applied directed evolution to TrpB from Pyrococcus furiosus and Thermotoga maritima to generate a suite of catalysts for the synthesis of previously intractable Trp analogues...
Year: 2016
Abstract:
We report that L-threonine may substitute for L-serine in the β substitution reaction of an engineered subunit of tryptophan synthase from Pyrococcus furiosus, yielding (2S,3S)-β-methyltryptophan (β=MeTrp) in a single step...
Year: 2015
Abstract:
We used directed evolution to explore allosteric regulation as a source of latent catalytic potential using the β-subunit of tryptophan synthase from Pyrococcus furiosus (PfTrpB)...