Aralez Bio

News, Events, and Announcments

Fundraising

We just closed a $12MM round of financing...

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Q1 2025 Newsletter

February 2025: Delve further into the world of noncanonical amino acids with Articles, Highlights, Events and Insights from the scientists and customers of Aralez Bio.

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Q2 Newsletter Just Released

July 2024: Delve further into the world of noncanonical amino acids with Articles, Highlights, Events and Insights from the scientists and customers of Aralez Bio.

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IOPC June 2024

Tina Boville, Aralez Bio CEO and co-founder, is an invited speaker at this year's event. Her talk is titled, "Expanding the Universe of Noncanonical Amino Acids with Biocatalysis." Contact info@aralezbio.com if you'd like her to send you a copy of the presentation.

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Aralez Bio in the News - April 3, 2024

Aralez Bio was just featured in CHEManager International issue 01/2024. Tina and David were interviewed by this DACH-focused Wiley publication that gives strategic news to the chemical and pharmaceutical industries.

Go to the article

Q1 2024 Newsletter Just Released

Delve further into the world of noncanonical amino acids with Articles, Highlights, Events and Insights from the scientists and customers of Aralez Bio.

Download the newsletter

Aralez Bio in the Community - Oct. 2023

The City of San Leandro featured our CEO Tina Boville & our Lead Protein Engineer Ella Watkins-Dulaney in their Made in San Leandro: Celebrating Women in Manufacturing series.

Watch the video segment

Aralez Bio

 

Core Publications

Year: 2021

Abstract:

Here, we show that ketone-derived enolates can serve as nucleophiles in the TrpB reaction to achieve the asymmetric alkylation of ketones, an outstanding challenge in synthetic chemistry...

link to article

2021---Watkins-Dulaney-et-al.---Asymmetric-Alkylation-of-Ketones-Catalyzed-by-Engineered-TrpB

 

Year: 2020

Abstract:

This review examines the long and storied career of TrpS from the perspective of its application in ncAA synthesis and biocatalytic cascades.

link to article

 

2020---Watkins-Dulaney-et-al.-Tryptophan-Synthase--Biocatalyst-Extraordinaire

 

Year: 2020

Abstract:

Using a continuous directed evolution platform called OrthoRep, we rapidly evolve the Thermotoga maritima tryptophan synthase β-subunit (TmTrpB)... 

link to article

2020---Rix-et-al.---Scalable-continuous-evolution-for-the-generation-of-diverse-enzyme-variants-encompassing-promiscuous-activities

 

Year: 2019

Abstract:

Using an engineered tryptophan synthase β-subunit (TrpB), stereochemically pure AzAla can be synthesized at scale starting from commercially available azulene and L-serine....

link to article

2019--Watkins-et-al.--Direct-enzymatic-synthesis-of-a-deep-blue-fluorescent-noncanonical-amino-acid-from-azulene-and-serine--ChemBioChem

 

Year: 2019

Abstract:

Here we report the use of nitroalkanes as a structurally versatile class of nucleophilic substrates for C−C bond formation catalyzed by variants of the β-subunit of tryptophan synthase (TrpB)...

link to article

2019---Romney-et-al.--Nitroalkanes

 

Year: 2019

Abstract:

Here we employ directed evolution to engineer TrpB to accept 3-substituted oxindoles and form C–C bonds leading to new quaternary stereocenters...

link to article

2019---Dick-et-al.---Tailoring-Tryptophan-Synthase-TrpB-for-Selective-Quarternary-Carbond-Bond-Formation

 

Year: 2018

Abstract:

Here, we show that directed evolution caused a gradual change in the rate-limiting step of the catalytic cycle...

link to article

2018---Buller-et-al.---Directed-evolution-mimics-allosteric-activation-by-stepwise-tuning-of-the-conformational-ensemble---Unknown

 

Year: 2018

Abstract:

Here, we describe the engineering of TrpB from Thermotoga maritima that improved synthesis of 4-CN-Trp from 24% to 78% yield...

link to article

2018---Boville-et-al.--Improved-Synthesis-of-4-Cyanotryptophan-and-Other-Tryptophan-Analogues-in-Aqueous-Solvent-Using-Variants-of-TrpB-from-Thermotoga-maritima

 

Year: 2018

Abstract:

Herein we report a stereoselective biocatalytic synthesis of beta-branched tryptophan analogues using PfTrpB7E6...

link to article

2018---Boville-et-al.---Engineered-biosynthesis-of-β-alkyl-tryptophan-analogs---Angewandte-Chemie-International-Edition

 

Year: 2018

Abstract:

...we briefly outline different enzyme engineering strategies and then discuss examples where engineering has generated new ‘ncAA synthases’ for efficient, environmentally benign production...

link to article

2018---Almhjell,-Boville,-Arnold---Engineering-enzymes-for-noncanonical-amino-acid-synthesis---Unknown

 

Year: 2017

Abstract:

We have applied directed evolution to TrpB from Pyrococcus furiosus and Thermotoga maritima to generate a suite of catalysts for the synthesis of previously intractable Trp analogues...

link to article

2017---Romney-et-al.---Unlocking-Reactivity-of-TrpB-A-General-Biocatalytic-Platform-for-Synthesis-of-Tryptophan-Analogues---Journal-of-t

 

Year: 2016

Abstract:

We report that L-threonine may substitute for L-serine in the β substitution reaction of an engineered subunit of tryptophan synthase from Pyrococcus furiosus, yielding (2S,3S)-β-methyltryptophan (β=MeTrp) in a single step...

link to article

2016---Herger-et-al.---Synthesis-of-β-Branched-Tryptophan-Analogues-Using-an-Engineered-Subunit-of-Tryptophan-Synthase---Journal-of-the

 

Year: 2015

Abstract: 

We used directed evolution to explore allosteric regulation as a source of latent catalytic potential using the β-subunit of tryptophan synthase from Pyrococcus furiosus (PfTrpB)...

link to article

2015---Buller-et-al.---Directed-evolution-of-the-tryptophan-synthase-β-subunit-for-stand-alone-function-recapitulates-allosteric-activat

 

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